Bacillus thermocatenulatus lipase: a thermoalkalophilic lipase with interesting properties.
نویسندگان
چکیده
Montero, S., Blanco, A., Virto, M. D., Landeta, L. C., Agud, I., Solozabel, R., Lascaray, J. M., De Renobles, M., Llama, M. J. and Serra, J. L. (1993) Enzyme Microb. Technol. 15,239-247 Huang, F.-C. and Ju, Y.-H. (1994) Biotechnol. Tech. 8,827-830 AIChE J. 35, 1551-1554 21 Bailie, P. M., McNerlan, S. E., Robinson, E. and Murphy, W. R. (1995) Trans. IChemE. 73, 71-76 22 Wehtje, E., Adlercreutz, P. and Mattiasson, B. (1992) Prog. Biotechnol. 8, 377-382 23 Bosley, J. A. and Peilow, A. D. (1991) European Patent Application EP 424,130 24 Macrae, A. R., Roehl, E. L. and Brand, H. M. (1984) Seifen Ole Fette Wasche 116,201-205 25 Quinlan, P. and Moore, S. (1993) Int. News Fats, Oils Relat. Mater. 4, 580-585
منابع مشابه
The Conserved Lid Tryptophan, W211, Potentiates Thermostability and Thermoactivity in Bacterial Thermoalkalophilic Lipases
We hypothesize that aggregation of thermoalkalophilic lipases could be a thermostability mechanism. The conserved tryptophans (W211, W234) in the lid are of particular interest owing to their previous involvements in aggregation and thermostability mechanisms in many other proteins. The thermoalkalophilic lipase from Bacillus thermocatenulatus (BTL2) and its mutants (W211A, W234A) were expresse...
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Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a the...
متن کاملPeriplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences
Efforts to express lipase in the periplasmic space of Escherichia coli have so far been unsuccessful andmost of the expressed recombinant lipases accumulate in the insoluble cell fraction. To evaluate the role ofnative and heterologous signal peptides in translocation of the lipase across the inner membrane of E. coli,the lipase gene (btl2) was cloned downstream of the native ...
متن کاملIsolation and Characterization of a New Thermoalkalophilic Lipase from Soil Bacteria
Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a the...
متن کاملConversion of Bacillus thermocatenulatus lipase into an efficient phospholipase with increased activity towards long-chain fatty acyl substrates by directed evolution and rational design.
The thermoalkalophilic lipase from Bacillus thermocatenulatus BTL2 exhibits a low phospholipase activity (lecithin/tributyrin ratio 0.03). A single round of random mutagenesis of the BTL2 gene followed by screening of 6000 transformants on egg-yolk plates identified three variants with 10-12-fold increased phospholipase activities, corresponding to lecithin/tributyrin ratios of 0.16-0.36. All v...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 25 1 شماره
صفحات -
تاریخ انتشار 1997